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G5035
Sigma
Granulocyte-Macrophage Colony-Stimulating Factor human
from human, recombinant, expressed in Escherichia coli, cell culture tested
| Synonym: | GM-CSF human |
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Analysis Note | The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells.1 |
| Biochem/physiol Actions | Granulocyte-macrophage colony stimulating factor is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity. |
| General description | Package size based on protein content |
| Physical form | Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution. |
Properties
| biological source | from human |
| recombinant | expressed in Escherichia coli |
| assay | ≥98% (SDS-PAGE and HPLC) |
| form | lyophilized powder |
| potency | ≤0.1 ng/mL EC50 |
| 0.01-0.1 ng/mL ED50/EC50 | |
| mol wt | mol wt 14.6 kDa |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
References
| Cited Reference | 1. Kitamura, T., et al., Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell Physiol. 140, 323-334, (1989) Abstract |
| reference | Nicola, N.A., Nicola, N.A., ed. Guidebook to Cytokines and Their Receptors NY, NY , (1994), 171 |
| Smith, L.R., et al. Immunogenetics 39, 80, (1994) | |
| Quesniaux, V.F.J., Jones, T.C. The Cytokine Handbook 3rd ed., San Diego, CA , (1998), 637 | |
| Callard, R., Gearing, A. The Cytokine Facts Book New York, NY , (1994) | |
| Kelso, A., Cytokines: principles and prospects. Cell Biol. 76, 300, (1998) | |
| Guthridge, M., Mechanism of activation of the GM-CF, IL-3, and IL-5 family of receptors. Stem Cells 16, 301, (1998) | |
| Heim, M., The Jak-STAT pathway: cytokine signaling from the receptor to the nucleus. J. Recept. Signal Transduct. Res. 19, 75, (1999) | |
| Skoda, R., Specificity of signaling by hematopoietic cytokin receptors: instructive versus permissive effects. J. Recept. Signal Transduct. Res. 19, 741, (1999) |
